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2 hasil
Subjek: Bacteria
DaftarKartu
ThesisOpen AccessMetadata saja

UJI EFEK ANTIBAKTERI EKSTRAK DAUN MANGROVE Rhizophora apiculata Blume TERHADAP BAKTERI Salmonella thypi

2022•Skripsi, Thesis, Disertasi, dan Tugas Akhir

ABSTRAK Uji Efek Antibakteri Ekstrak Daun Mangrove Rhizophora apiculata Blume Terhadap Bakteri Salmonella thypi Batu, S.F)* Rafael, A)** Bulu, I.N)** Tumbuhan mangrove merupakan salah satu ekosistem pantai yang mendominasi wilayah pantai suatu daerah dan berpotensi untuk dikembangkan dalam penanggulangan berbagai penyakit baik infeksi pada ikan budidaya maupun sebagai media pengobatan alami bagi manusia, karena senyawa metabolit sekunder yang terkandung didalamnya mampu berkhasiat sebagai antibakteri. Tujuan dari penelitian ini untuk mengetahui pengaruh ekstrak daun mangrove R. apiculata Blume terhadap pertumbuhan bakteri S. thypi dan konsentrasi optimum dari ekstrak daun mangrove R. apiculata Blume dalam menghambat pertumbuhan bakteri S. thypi. Metode yang digunakan dalam penelitian ini metode eksperimen laboratorium dengan 4 perlakuan yaitu konsentrasi 55 mg/mL, 60 mg/mL, 65 mg/mL, 70 mg/mL dan 1 kontrol tanpa pemberian ekstrak dengan 3 kali ulangan. Hasil pengamatan diameter zona hambat dianalisis secara statistik menggunakan analisis variansi (ANOVA) satu jalur dengan signifikansi 5%. Analisis statistik dilakukan menggunakan program SPSS versi 27.0. Hasil penelitian menunjukkan bahwa ekstrak daun mangrove R. apiculata Blume berpengaruh terhadap pertumbuhan bakteri S. thypi. Hal ini dapat dilihat dari konsentrasi yang digunakan mulai dari 55 mg/mL, 60 mg/mL, 65 mg/mL, 70 mg/mL memiliki rata-rata zona hambat yang berkisar antara 19 mm, 19 mm, 22 mm dan 26 mm. Dengan demikian semakin tinggi konsentrasi ekstrak yang digunakan, maka semakin besar daya hambat yang terbentuk. Kata kunci : Rhizophora apiculata Blume , Salmonella thypi, Antibakteri Keterangan : *) : Peneliti **) : Pembimbing ABSTRACT Antibacterial Effect Test Of Rhizophora apiculata Blume Mangrove Leaf Extract Against Salmonella thypi Bacteria Batu, S.F)* Rafael, A)** Bulu, I.N)** Mangrove plant is one of the coastal ecosystems that dominates the coastal area and has the potential to be developed in the prevention of various diseases both infections in aquaculture and as a natural treatment medium for humans, because the secondary metabolite compound contained can be efficacious as an anti-bacterial. The purpose of this study was to determine the effect of R. apicullata Blume mangrove bark extract against the growth of S. thypi and the optimum concentration of R. apiculata Blume mangrove bark extract in inhibiting the growth of S. thypi bacteria. The method used in this study is a laboratory experimental method with 4 treatments namely a concentration of 55 mg/mL, 60 mg/mL, 65 mg/mL, 70 mg/mL and 1 control without administration of extract with 3 replications. Observation result of inhibition zone diameters were statistically analyzed using a one-way analysis was performed using the SPSS program version 27.0. The results showed that the rough extract of R. apiculata Blume mangrove leaf affected the growth of S.thypi bacteria. This can be seen from the concentrations used starting from 55 mg/mL, 60 mg/mL, 65 mg/mL and 70 mg/mL having average inhibition zones ranging from 19 mm, 19 mm, 22 mm and 26 mm. thus the higher the concentration of the extract used, the greater the inhibition formed. Keywords : Rhizophora apiculata Blume , Salmonella thypi, Antibacterial Description : *) : Researcher **) : Mentor

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ThesisOpen AccessMetadata saja

ISOLASI DAN SELEKSI BAKTERI SIMBION SPONS (PORIFERA: DEMOSPONGIAE) YANG MENGHASILKAN ENZIM EKSTRASELULER DARI PANTAI TABLOLONG

2021•Skripsi, Thesis, Disertasi, dan Tugas Akhir

Marine biota has many benefits for human life. Sponges are a species of marine biota that can be used as a producer of antimicrobial compounds. The bacteria found in sponges have an important role in the continuity of life in the sea. This lifestyle between sponges and bacteria forms a symbiosis that forms secondary metabolite compounds and enzymes, especially extracellular enzymes (amylase, protease, cellulase and lipase). This study aims to determine how to isolate sponge symbiotic bacteria and identify spongy symbiotic bacteria. The method used is purposive to take sponges. Characterization of bacteria was carried out based on morphology and gram staining. Enzymatic bacterial selection was carried out by testing the activity of the amylase enzyme (soluble starch), lipase enzyme (Tween 80), protease enzyme (skim milk) and cellulose enzyme (carboxyl methyl cellulose). Data analysis was performed in a qualitative descriptive manner by measuring the clear zone in the extracellular enzyme test results. Meanwhile, other research parameters measured in this study were temperature, salinity and pH. The results showed that out of 47 isolated bacteria and 33 of them had extracellular enzymes with 10 bacteria had amylase enzymes, 27 bacteria had lipase enzymes, 2 bacteria had protease enzymes and 1 isolate had cellulose enzymes.

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